Phenylcoumaran benzylic ether reductase, a prominent poplar xylem protein, is strongly associated with phenylpropanoid biosynthesis in lignifying cells.
Identifieur interne : 004783 ( Main/Exploration ); précédent : 004782; suivant : 004784Phenylcoumaran benzylic ether reductase, a prominent poplar xylem protein, is strongly associated with phenylpropanoid biosynthesis in lignifying cells.
Auteurs : K. Vander Mijnsbrugge [Belgique] ; H. Beeckman ; R. De Rycke ; M. Van Montagu ; G. Engler ; W. BoerjanSource :
- Planta [ 0032-0935 ] ; 2000.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Arbres.
- immunologie : Oxidoreductases, Protéines recombinantes.
- métabolisme : Arbres, Lignanes, Oxidoreductases, Phénylpropionates.
- Production d'anticorps, Technique d'immunofluorescence.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Oxidoreductases, Recombinant Proteins.
- chemical , metabolism : Lignans, Oxidoreductases, Phenylpropionates.
- enzymology : Trees.
- metabolism : Trees.
- Antibody Formation, Fluorescent Antibody Technique.
Abstract
It has previously been shown (D.R. Gang et al., 1999, J Biol Chem 274: 7516-7527) that the most abundant protein in the secondary xylem of poplar (Populus trichocarpa cv. 'Trichobel') is a phenylcoumaran benzylic ether reductase (PCBER), an enzyme involved in lignan synthesis. Here, the distribution and abundance of PCBER in poplar was studied at both the RNA and protein level. The cellular expression pattern was determined by immunolocalization of greenhouse-grown plants as well as of a field-grown poplar. Compared to other poplar tissues, PCBER is preferentially produced in the secondary xylem of stems and roots and is associated with the active growth period. The protein is present in all cells of the young differentiating xylem, corresponding to the zone of active phenylpropanoid metabolism and lignification. In addition, PCBER is located in young differentiating phloem fibers, in xylem ray parenchyma, and in xylem parenchyma cells at the growth-ring border. Essentially the same expression pattern was observed in poplars grown in greenhouses and in the field. The synthesis of PCBER in phenylpropanoid-synthesizing tissues was confirmed in a bending experiment. Induction of PCBER was observed in the pith of mechanically bent poplar stems, where phenylpropanoid metabolism is induced. These results indicate that the products of PCBER activity are synthesized mainly in lignifying tissues, suggesting a role in wood development.
DOI: 10.1007/s004250000326
PubMed: 11030549
Affiliations:
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Vander Mijnsbrugge</name><affiliation wicri:level="4"><nlm:affiliation>Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent</wicri:regionArea><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Beeckman, H" sort="Beeckman, H" uniqKey="Beeckman H" first="H" last="Beeckman">H. Beeckman</name></author><author><name sortKey="De Rycke, R" sort="De Rycke, R" uniqKey="De Rycke R" first="R" last="De Rycke">R. De Rycke</name></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Engler, G" sort="Engler, G" uniqKey="Engler G" first="G" last="Engler">G. Engler</name></author><author><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:11030549</idno><idno type="pmid">11030549</idno><idno type="doi">10.1007/s004250000326</idno><idno type="wicri:Area/Main/Corpus">004826</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004826</idno><idno type="wicri:Area/Main/Curation">004826</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004826</idno><idno type="wicri:Area/Main/Exploration">004826</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Phenylcoumaran benzylic ether reductase, a prominent poplar xylem protein, is strongly associated with phenylpropanoid biosynthesis in lignifying cells.</title><author><name sortKey="Vander Mijnsbrugge, K" sort="Vander Mijnsbrugge, K" uniqKey="Vander Mijnsbrugge K" first="K" last="Vander Mijnsbrugge">K. Vander Mijnsbrugge</name><affiliation wicri:level="4"><nlm:affiliation>Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent</wicri:regionArea><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Beeckman, H" sort="Beeckman, H" uniqKey="Beeckman H" first="H" last="Beeckman">H. Beeckman</name></author><author><name sortKey="De Rycke, R" sort="De Rycke, R" uniqKey="De Rycke R" first="R" last="De Rycke">R. De Rycke</name></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Engler, G" sort="Engler, G" uniqKey="Engler G" first="G" last="Engler">G. Engler</name></author><author><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody Formation (MeSH)</term><term>Fluorescent Antibody Technique (MeSH)</term><term>Lignans (metabolism)</term><term>Oxidoreductases (immunology)</term><term>Oxidoreductases (metabolism)</term><term>Phenylpropionates (metabolism)</term><term>Recombinant Proteins (immunology)</term><term>Trees (enzymology)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (enzymologie)</term><term>Arbres (métabolisme)</term><term>Lignanes (métabolisme)</term><term>Oxidoreductases (immunologie)</term><term>Oxidoreductases (métabolisme)</term><term>Phénylpropionates (métabolisme)</term><term>Production d'anticorps (MeSH)</term><term>Protéines recombinantes (immunologie)</term><term>Technique d'immunofluorescence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Oxidoreductases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignans</term><term>Oxidoreductases</term><term>Phenylpropionates</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arbres</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Oxidoreductases</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arbres</term><term>Lignanes</term><term>Oxidoreductases</term><term>Phénylpropionates</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Antibody Formation</term><term>Fluorescent Antibody Technique</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Production d'anticorps</term><term>Technique d'immunofluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It has previously been shown (D.R. Gang et al., 1999, J Biol Chem 274: 7516-7527) that the most abundant protein in the secondary xylem of poplar (Populus trichocarpa cv. 'Trichobel') is a phenylcoumaran benzylic ether reductase (PCBER), an enzyme involved in lignan synthesis. Here, the distribution and abundance of PCBER in poplar was studied at both the RNA and protein level. The cellular expression pattern was determined by immunolocalization of greenhouse-grown plants as well as of a field-grown poplar. Compared to other poplar tissues, PCBER is preferentially produced in the secondary xylem of stems and roots and is associated with the active growth period. The protein is present in all cells of the young differentiating xylem, corresponding to the zone of active phenylpropanoid metabolism and lignification. In addition, PCBER is located in young differentiating phloem fibers, in xylem ray parenchyma, and in xylem parenchyma cells at the growth-ring border. Essentially the same expression pattern was observed in poplars grown in greenhouses and in the field. The synthesis of PCBER in phenylpropanoid-synthesizing tissues was confirmed in a bending experiment. Induction of PCBER was observed in the pith of mechanically bent poplar stems, where phenylpropanoid metabolism is induced. These results indicate that the products of PCBER activity are synthesized mainly in lignifying tissues, suggesting a role in wood development.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11030549</PMID><DateCompleted><Year>2001</Year><Month>02</Month><Day>08</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>211</Volume><Issue>4</Issue><PubDate><Year>2000</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Phenylcoumaran benzylic ether reductase, a prominent poplar xylem protein, is strongly associated with phenylpropanoid biosynthesis in lignifying cells.</ArticleTitle><Pagination><MedlinePgn>502-9</MedlinePgn></Pagination><Abstract><AbstractText>It has previously been shown (D.R. Gang et al., 1999, J Biol Chem 274: 7516-7527) that the most abundant protein in the secondary xylem of poplar (Populus trichocarpa cv. 'Trichobel') is a phenylcoumaran benzylic ether reductase (PCBER), an enzyme involved in lignan synthesis. Here, the distribution and abundance of PCBER in poplar was studied at both the RNA and protein level. The cellular expression pattern was determined by immunolocalization of greenhouse-grown plants as well as of a field-grown poplar. Compared to other poplar tissues, PCBER is preferentially produced in the secondary xylem of stems and roots and is associated with the active growth period. The protein is present in all cells of the young differentiating xylem, corresponding to the zone of active phenylpropanoid metabolism and lignification. In addition, PCBER is located in young differentiating phloem fibers, in xylem ray parenchyma, and in xylem parenchyma cells at the growth-ring border. Essentially the same expression pattern was observed in poplars grown in greenhouses and in the field. The synthesis of PCBER in phenylpropanoid-synthesizing tissues was confirmed in a bending experiment. Induction of PCBER was observed in the pith of mechanically bent poplar stems, where phenylpropanoid metabolism is induced. These results indicate that the products of PCBER activity are synthesized mainly in lignifying tissues, suggesting a role in wood development.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vander Mijnsbrugge</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Beeckman</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>De Rycke</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Van Montagu</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Engler</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>W</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017705">Lignans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010666">Phenylpropionates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.97.-</RegistryNumber><NameOfSubstance UI="C118150">phenylcoumaran benzylic ether reductase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000917" MajorTopicYN="N">Antibody Formation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017705" MajorTopicYN="N">Lignans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010666" MajorTopicYN="N">Phenylpropionates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>10</Month><Day>13</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>3</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>10</Month><Day>13</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11030549</ArticleId><ArticleId IdType="pii">10.1007/s004250000326</ArticleId><ArticleId IdType="doi">10.1007/s004250000326</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Beeckman, H" sort="Beeckman, H" uniqKey="Beeckman H" first="H" last="Beeckman">H. Beeckman</name><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name><name sortKey="De Rycke, R" sort="De Rycke, R" uniqKey="De Rycke R" first="R" last="De Rycke">R. De Rycke</name><name sortKey="Engler, G" sort="Engler, G" uniqKey="Engler G" first="G" last="Engler">G. Engler</name><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Vander Mijnsbrugge, K" sort="Vander Mijnsbrugge, K" uniqKey="Vander Mijnsbrugge K" first="K" last="Vander Mijnsbrugge">K. Vander Mijnsbrugge</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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